Advantages of long-read sequencing for mRNA profiling of biological systems

mRNA sequencing (mRNA-Seq) evaluates the transcriptome of disease states and biological processes. mRNA-Seq is a precise method for measuring gene expression (Eichler, 2017). Unlike small reads typically produced by next-generation transcriptome sequencing, long reads help detect full-length transcripts, gene fusions, and allele-specific expression.

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Tools to evaluate the quality of third-generation/long-read sequencing data

Third-generation sequencing, also known as long-read sequencing, uses technologies developed by Pacific Biosciences (PacBio) and Oxford Nanopore Technologies to elucidate the sequence of a DNA molecule. Several software tools are available in the public domain to evaluate the quality of sequencing results from third-generation sequencers.

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Aligners for long reads produced by third-generation sequencers

The third-generation sequencing technologies have rendered some read alignment tools irrelevant but still provided the background for new tools better suited for modern sequencing technologies.

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Adverse drug reactions (ADRs) caused by genomic variations through modulation of Pharmacokinetics and Pharmacodynamics of a Drug

How does genetic make-up determine the possibility of developing ADRs to medications?

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